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High Risk Human PapillomaViral infections & the risk of developing oraland pharyngeal cancer: Immuno and Molecular epidemiologic evidence from Sri Lanka

NRC Grant: 13-130

Prof. P.V. Udagama
Dept. of Zoology
Faculty of Science
University of Colombo
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Area of Research: Biotechnology & Medical Science
Status: Ongoing

 

Objectives

  1. a) To establish the association between high risk HPV infections and oral and pharyngeal cancer in Sri Lanka, using prevalence of appropriate serologic markers and molecular detection of HPV DNA to confirm infection with high-risk HPV in OPC patients. b) Production of relevant HPV antigens (HPV16, HPV18, HPV31, HPV33 and HPV35 assessing L1, E6 and E7) in the form of recombinant proteins. c) To collect biological samples (blood for serum, oral exfoliated cells and biopsy specimens) and to accrue demographic, socio-economic and medical information of test subjects to elucidate plausible aetiologic factors associated with OPC. The test population will include (i) newly diagnosed OPC patients from the National Cancer Institute, (ii) clinically diagnosed HPV positive patients from the STD clinic, National Hospital, Sri Lanka and (iii) age and gender matched healthy non-cancer control population (with a smoking subgroup). d) To accrue demographic, socio-economic and medical information of each subject to elucidate and establish other plausible aetiologic factors associated with OPC. e) To assay for HPV specific IgG antibodies for HPV16, HPV18, HPV31, HPV33 and HPV35 assessing L1, E6 and E7 in test and control serum samples using in-house developed, validated indirect ELISAs. f) Molecular detection of HPV DNA using fluorescent in-situ hybridization (FISH). g) Use of logistic regression models to investigate the association between HPV status and OPC in relation to HPV DNA evidence, IgG responses to HPV, other established major risk factors, and histopathologic characteristics of OPC patients.

 

 

You are here: Home Grants - 2013 High Risk Human PapillomaViral infections & the risk of developing oraland pharyngeal cancer: Immuno and Molecular epidemiologic evidence from Sri Lanka