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Polyphenols from refuse tea and green tea dust for clinical applications: enhancing activity of antibiotics against common drug-resistant pathogens and inhibition of biofilm formation

NRC Grant: 12-114

Prof. B.M.R. Bandara
Dept. of Chemistry
Faculty of Science
University of Peradeniya
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Area of Research: Medical Sceince
Status: Ongoing

 

Objectives

  1. a) To isolate catechin and proanthocyanidin fractions from green tea dust and theaflavin and thearubigin fractions from refuse tea and black tea, and record the high performance liquid chromatography (HPLC) profile and Fourier-Transform Infrared spectrum of each fraction. b) To evaluate the specific antimicrobial activity of polyphenol fracions (catechins, proanthocyanidins, theaflavins and thearubigins) individually and in combination against a sufficient number of drug-sensitive and -resistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Candida species. c) To determine MICs of antibiotics and antimycotics for drug-resistant strains of S. aureus, P. aeruginosa and Candida species incubated with polyphenol fractions individually and in combination. d) To test the inhibitory effects of polyphenol fractions on the development in three stages (adhesion, propagation and maturation) of biofilms from S. aureus, P. aeruginosa and Candida species. e) To test the inhibitory effects of polyphenol fractions together with antibiotics and antimycotics on the development in three stages (adhesion, propagation and maturation) of biofilms from S. aureus, P. aeruginosa and Candida species.

 

 

You are here: Home Grants - 2012 Polyphenols from refuse tea and green tea dust for clinical applications: enhancing activity of antibiotics against common drug-resistant pathogens and inhibition of biofilm formation