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Development of an optical whole cell microalgal-cyanobacterial array biosensor to assess the bioavailable toxic heavy metals in aquatic systems

NRC Grant: 12-092

 

12-092

Dr. I.V.N. Rathnayake
Dept. of Microbiology
Faculty of Science
University of Kelaniya
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Area of Research: Natural Resources & Water Management
Status: Ongoing

 

Objectives

  1. a) Isolation and purification of cyanobacteria and microalgae from the selected lentic and lotic water bodies in the Western province those are polluted with industrial and domestic waste discharges will be carried out since there are no collection of cyanobacteria and algae maintained in the department in this regard. b) Evaluation of the heavy metal tolerance by isolated organisms in single metal solutions as well as metal mixtures in response to growth retardation, and effect on chlorophyll fluorescence will be carried out to develop dose response relationships between the organisms and the respective metals/ mixtures of metals under laboratory conditions to select the most suitable cyanobacteria and the microalgae to be developed into the biosensor array. c) Once the Identification of the selected organisms are carried out using standard microbiological identification protocols, the cyanobacteria and microalgae will be immobilized in a suitable matrix to construct the biosensor array, and optimization of the operational conditions will be performed. d) Validation of the biosensor array using environmental samples will be carried out to evaluate the suitability of the biosensor to be used in the monitoring the risk assessment of aquatic systems for bioavailable heavy metals.

 

 

You are here: Home Grants - 2012 Development of an optical whole cell microalgal-cyanobacterial array biosensor to assess the bioavailable toxic heavy metals in aquatic systems